Toward complete carbon utilization: Improved methane yield from formate and hydrogen co-feeding through constitutive formate dehydrogenase-gene expression in Methanothermobacter thermautotrophicus ΔH
完全な炭素利用に向けて：ギ酸と水素の同時供給によるメタン収率向上（Methanothermobacter thermautotrophicus ΔHにおける構成的ギ酸脱水素酵素遺伝子発現） (AI 翻訳)
Aaron Zipperle, Largus T. Angenent, Gerben R. Stouten, Bastian Molitor
🤖 gxceed AI 要約
日本語
本研究は、CCU技術において重要な中間体であるギ酸をメタンに変換する微生物の遺伝子工学を報告。水素共存下でもギ酸を利用できる組み換え菌を開発し、炭素利用率を76.6%まで向上させた。水素による代謝制御を回避する戦略を示し、ギ酸からのメタン生成の実用化に貢献する。
English
This study reports genetic engineering of a methanogen to convert formate, a key CCU intermediate, to methane. The engineered strain simultaneously utilizes formate and hydrogen, achieving 76.6% carbon utilization, bypassing hydrogen-induced regulation. It identifies a promising biocatalyst for formate-to-methane conversion, advancing carbon capture and utilization technologies.
Unofficial AI-generated summary based on the public title and abstract. Not an official translation.
📝 gxceed 編集解説 — Why this matters
日本のGX文脈において
日本のGX政策ではCCUS技術の開発が重要視されており、本研究成果はメタネーション技術の効率向上に寄与する可能性がある。ただし、実用化にはスケールアップや経済性の検証が必要であり、現時点では基礎研究段階の知見として位置づけられる。
In the global GX context
Globally, CCUS is a key pillar of decarbonization strategies. This work advances biological methanation, offering a potential route to convert captured CO2 into methane, a valuable energy carrier. It contributes to the growing field of power-to-gas and circular carbon economy, though industrial deployment remains distant.
👥 読者別の含意
🔬研究者:Provides a novel genetic strategy to overcome metabolic limitations in formate-to-methane conversion, relevant for bio-based CCU research.
🏢実務担当者:Offers a potential biocatalyst for future industrial methanation processes, but requires further scale-up and economic assessment.
🏛政策担当者:Highlights the potential of biological CCU technologies, supporting R&D funding and pilot projects in the context of national decarbonization strategies.
📄 Abstract（原文）
Formate is emerging as a relevant intermediate in carbon capture and utilization technologies. However, its low energy density limits its value as an energy carrier. Some hydrogenotrophic methanogens can reduce formate to the established energy carrier methane. The stoichiometric limitation of formate disproportionation is that 75 % of the carbon is released as carbon dioxide, and achieving a complete carbon utilization requires co-feeding hydrogen. However, hydrogen-dependent genetic regulation of formate metabolism inhibits simultaneous formate and hydrogen utilization in hydrogenotrophic methanogens. Here, we compared the catalytic performance of the genetically modified strain Methanothermobacter thermautotrophicus ΔH pMVS1111A:PhmtB-fdh Z-245 (pFdh) with M. thermautotrophicus Z-245 using continuous cultivation at different hydrogen concentrations . While M. thermautotrophicus Z-245 is natively formatotrophic, M. thermautotrophicus ΔH (pFdh) was engineered to enable formate utilization via plasmid-borne expression of a formate dehydrogenase-gene cassette. We found that M. thermautotrophicus ΔH (pFdh) can simultaneously utilize formate and hydrogen. It continuously consumed formate at a dissolved hydrogen concentration of 0.069 ± 0.004 mM, enabling a 76.6 % ± 0.9 % carbon utilization. M. thermautotrophicus Z-245 showed declining formate consumption as the dissolved hydrogen concentration increased toward approximately 0.02 mM and reached a maximum stable carbon utilization of 36.2 % ± 0.2 %. These results suggest that M. thermautotrophicus ΔH (pFdh) largely bypasses hydrogen-dependent transcriptional control of formate metabolism; however, it still faces redox-related metabolic limitations at dissolved hydrogen concentrations above 0.32 mM. Overall, the findings reveal a potential strategy to circumvent hydrogen-induced regulation of formate metabolism and identify M. thermautotrophicus ΔH (pFdh) as a promising biocatalyst for formate-to-methane conversion.
🔗 Provenance — このレコードを発見したソース
- openalex https://doi.org/10.1016/j.biortech.2026.135368first seen 2026-08-09 05:12:55
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